Research Services

Devoted to Personalized Cancer Medicine and Next-Generation Sequencing

Geneseeq’s North American headquarters is in the MaRS Discovery District in Toronto, Canada. Outside of Canada, we have an established bioinformatics division at Stanford University. Our laboratories have successfully passed China’s National Center of Clinical Laboratories (NCCL), External Quality Assessment (EQA), College of American Pathologist (CAP) PT, European Molecular Genetics Quality Network (EQMN) PT and received third-party medical laboratory and clinical PCR amplification laboratory qualifications.

Our high-performance computer (HPC) consists of 120 computer nodes, 10 fat nodes and 6 high performance parallel super storage units. It has a compute capacity of up to 98.3 tFLOPS, an internal memory of 27TB and a total storage of 30PB. This ensures efficient data processing and safe storage.

Whole Genome re-Sequencing, WGS

  • WGS compares the individual’s genomic sequence to a reference genome to identify genetic alterations specific to the individual – usually in the form of single nucleotide polymorphisms (SNPs), insertion/deletion (Indels), structural variation (SV) and copy number variation (CNV).
  • WGS results are widely used in mutation detection, genetic map construction, functional gene mining, population evolution and artificial selection.

Metagenomics Sequencing

  • Metagenomics sequencing refers to the comprehensive sampling of all organisms recovered directly from the environment.
  • Through metagenomics sequencing, microbes can be identified and quantified in various environments. It is also an opportunity to study unculturable microorganisms and reveal their potential biological utility.

Whole Exome Sequencing, WES

  • WES is one of the most common targeted sequencing methods. The exome – protein coding region of the genome – represents <2% of the genome and contains ~85% of disease-causing genetic alterations.
  • Compared to WGS, WES is inexpensive and effective in detecting SNPs, Indels, SVs and CNVs across coding regions. It facilitates the discovery of novel genomic variations and investigation of medical relevant genomic regions.

Transcriptome Sequencing

  • Transcriptome sequencing uses high-throughput technology to rapidly identify the presence and quantity of RNA under a given condition.
  • This sequencing method is useful for investigating gene function, signaling pathways, alternative splicing and new transcripts. 

Long non-coding RNA Sequencing, lncRNA-Seq

  • lncRNA is a class of RNA with more than 200 nucleotides in length and does not encode proteins. It has been implicated in human pathology by regulating gene expression through epigenetic regulation, transcriptional regulation, post-transcriptional regulation, and translational regulation.
  • lncRNA sequencing captures 17Mb of human lncRNA and determines their expression level. It is useful in developmental regulation research, identifying tissue- and cell-type specificity, and disease related lncRNA.  

Small RNA Sequencing

  • Small RNAs are a class of regulatory molecules such as miRNA, piRNA and siRNA that are 18-30 nucleotides in length.
  • They act in gene silencing, post-transcriptional regulation of gene expression, regulating cell growth, differentiation and other important biological processes. Small RNA sequencing is used to sequence all small RNAs in the sample and determine the level of expression. 

Circular RNA Sequencing, circRNA-Seq

  • CircRNA is a non-coding RNA subtype that forms a closed ring structure and lacks a 5’ cap and 3’ poly(a) tail. It mainly acts as a miRNA inhibitor, increasing target gene expression by binding to miRNA through its miRNA binding site.
  • CircRNA sequencing quantifies the expression of circRNAs and identify new circRNAs for the purpose of exploring developmental regulation, tissue- and cell-type specificity, and disease related CircRNA.

Whole Genome Bisulfite Sequencing, WGBS

  • WGBS detects DNA methylation of single cytosine bases by treating DNA with bisulfite prior to sequencing. It functions at the single-base level to identify and analyze the methylation of each C-base to construct a genome wide methylation map. WGBS is the gold standard for methylation analysis.
  • DNA methylation helps regulate genes: it maintains chromosome structure; X chromosome inactivation, genetic imprinting, embryonic development and regular cell body processes.

Chromatin Immunoprecipitation Sequencing, ChIP-Seq

  • ChIP-Seq is widely used to identify the binding loci of transcription factors or other DNA-binding proteins on DNA.
  • It produces an epigenetic modification profile which can be used to infer expression or chromatin structure changes of different tissue types or cells under different growth conditions.